Journal: Oncology Letters

Article Title: Notch pathway inhibitor DAPT accelerates in vitro proliferation and adipogenesis in infantile hemangioma stem cells

doi: 10.3892/ol.2021.13115

Figure Lengend Snippet: Primer sequences used for reverse transcription-quantitative PCR.

Article Snippet: Additional cultures were prepared and protein samples were collected to evaluate the effects of DAPT treatment on the expression levels of β-actin (dilution, 1:500; cat. no. 3700; Cell Signaling Technology, Inc.), PPARγ (dilution, 1:500; cat. no. BS-0530R-2; BIOSS), C/EBPα (dilution, 1:1,000; cat. no. BS-1630R; BIOSS), C/EBPβ (dilution, 1:1,000; cat. no. BS-1396R; BIOSS), adiponectin (dilution, 1:1,000; cat. no. BS-0471R; BIOSS) and IGF-1 (dilution, 1:1,000; cat. no. BS-0014R; BIOSS).

Techniques: Sequencing

Journal: Oncology Letters

Article Title: Notch pathway inhibitor DAPT accelerates in vitro proliferation and adipogenesis in infantile hemangioma stem cells

doi: 10.3892/ol.2021.13115

Figure Lengend Snippet: Exposure to DAPT promotes adipogenesis and hemangioma stem cell expression of adiponectin, C/EBPα, C/EBPβ and PPARγ. Expression levels of (A) adiponectin, (B) C/EBPα, (C) C/EBPβ, (D) PPARγ and (E) IGF-1. In addition, expression was evaluated using (F) western blotting and semi-quantified using β-actin as the loading control. The band densities of (G) adiponectin, (H) C/EBPα, (I) C/EBPβ, (J) PPARγ and (K) IGF-1 were analyzed using ImageJ software. n=3. ns, P>0.05, *P<0.05, **P<0.01 (n=3). ADIP, adipogenic medium; ADIP+DAPT, adipogenic medium containing DAPT; ADIP+DMSO, adipogenic medium containing DMSO; C/EBPα, CCAAT/enhancer-binding protein α; C/EBPβ, CCAAT/enhancer-binding protein β; CON, control; DAPT, N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester; IGF-1, insulin-like growth factor 1; ns, not significant; PPARγ, peroxisome proliferator-activated receptor-γ.

Article Snippet: Additional cultures were prepared and protein samples were collected to evaluate the effects of DAPT treatment on the expression levels of β-actin (dilution, 1:500; cat. no. 3700; Cell Signaling Technology, Inc.), PPARγ (dilution, 1:500; cat. no. BS-0530R-2; BIOSS), C/EBPα (dilution, 1:1,000; cat. no. BS-1630R; BIOSS), C/EBPβ (dilution, 1:1,000; cat. no. BS-1396R; BIOSS), adiponectin (dilution, 1:1,000; cat. no. BS-0471R; BIOSS) and IGF-1 (dilution, 1:1,000; cat. no. BS-0014R; BIOSS).

Techniques: Expressing, Western Blot, Software, Binding Assay

Journal: Cancer Medicine

Article Title: Exploring the Relationship Between Adipocytokines and Endometrial Cancer: Identifying Correlations With Clinico‐Pathological Prognostic Factors

doi: 10.1002/cam4.71007

Figure Lengend Snippet: (A, B) Bar‐graph demonstrating the expression of (A) biomarkers and (B) their receptors in endometrial cancer tissue ( n = 39). X axis‐ biomarkers (A), receptors (B). Y axis–expressions of biomarkers as fold change over benign endometrial calibrator sample (assigned value = 1, for comparison with the cancer samples). The error bars represent standard errors of mean. 1 (C) Heat map demonstrating the expression of all 6 biomarkers and their receptors in endometrial cancer tissues ( n = 39). AdipoQ, adiponectin; ADIPOR1/R2, adiponectin receptors; OBR, leptin receptor; TNF1A, TNFRSF1A; TNF1B, TNFRSF1B (TNF receptors). Gene expressions are plotted on the Y ‐axis against the individual patients on the X ‐axis. Gene expressions are plotted as fold changes over a single pooled calibrator sample value. The color scale ranges from black (lowest value) to white (median) to yellow (highest value).

Article Snippet: The antibodies used were: ADIPOR1 (Proteintech, Manchester, UK, #66619‐1‐Ig; anti‐mouse antibody) and ADIPOR2 (Abcam, Cambridge, UK, #ab223752; anti‐rabbit antibody).

Techniques: Expressing, Comparison

Journal: Cancer Medicine

Article Title: Exploring the Relationship Between Adipocytokines and Endometrial Cancer: Identifying Correlations With Clinico‐Pathological Prognostic Factors

doi: 10.1002/cam4.71007

Figure Lengend Snippet: Heat map comparing the expression of (A) adiponectin, leptin and their receptors in endometrial cancer tissue and parametrial adipose tissue ( n = 39) (B) all 6 biomarkers and their receptors in endometrial cancer tissue and lymph nodes ( n = 12). AdipoQ, adiponectin; AdipoR1/R2, adiponectin receptors; ObR, leptin receptor; TNF1A, TNFRSF1A; TNF1B, TNFRSF1B (TNF receptors). Gene expressions are plotted on the Y ‐axis against the individual patients on the X ‐axis. Gene expression plotted as fold changes over a single pooled calibrator sample. The color scale ranges from black (lowest value) to white (median) to yellow (highest value).

Article Snippet: The antibodies used were: ADIPOR1 (Proteintech, Manchester, UK, #66619‐1‐Ig; anti‐mouse antibody) and ADIPOR2 (Abcam, Cambridge, UK, #ab223752; anti‐rabbit antibody).

Techniques: Expressing, Gene Expression

Journal: Cancer Medicine

Article Title: Exploring the Relationship Between Adipocytokines and Endometrial Cancer: Identifying Correlations With Clinico‐Pathological Prognostic Factors

doi: 10.1002/cam4.71007

Figure Lengend Snippet: Illustration of chromogenic 3,3′‐Diaminobenzidine (DAB) staining for ADIPOR1 (A) and ADIPOR2 (B) receptor in endometrial cancer tissue and benign tissues. Image taken at 10× magnification, Scalebar = 100 μm.

Article Snippet: The antibodies used were: ADIPOR1 (Proteintech, Manchester, UK, #66619‐1‐Ig; anti‐mouse antibody) and ADIPOR2 (Abcam, Cambridge, UK, #ab223752; anti‐rabbit antibody).

Techniques: Staining

Journal: Scientific reports

Article Title: Re-evaluation of serum leptin and adiponectin concentrations normalized by body fat mass in patients with rheumatoid arthritis.

doi: 10.1038/s41598-020-73068-2

Figure Lengend Snippet: Figure 1. Correlation of serum leptin and adiponectin concentrations with body fat mass. (a) shows the relationship between serum leptin and body fat mass in 136 patients with RA (filled circle) and 78 controls (white circle). The right figure demonstrates the comparison of serum leptin concentrations (mean ± SD) between obese (BMI ≥ 25, n = 59) and non-obese (BMI < 25, n = 155) subjects. (b) shows the relationship between serum adiponectin and body fat mass in 136 patients with RA (filled circle) and 78 controls (white circle). The right figure demonstrates the comparison of serum adiponectin concentrations between obese and non-obese subjects. Pearson’s correlation coefficient and student’s t test were used for the statistical analyses. P < 0.05 was considered statistically significant.

Article Snippet: EIA for adiponectin was performed in a similar manner, using anti-human adiponectin antibodies (10-7816 as the detection antibody and 10-7815 as the capture antibody) purchased from Fitzgerald Industries International (Acton, MA, USA).

Techniques: Comparison

Journal: Scientific reports

Article Title: Re-evaluation of serum leptin and adiponectin concentrations normalized by body fat mass in patients with rheumatoid arthritis.

doi: 10.1038/s41598-020-73068-2

Figure Lengend Snippet: Figure 2. Comparison of serum leptin and adiponectin concentrations between patients with RA and controls. Serum leptin concentrations expressed by raw data (a), those corrected by BMI (b) and those corrected by body fat mass (c); and serum adiponectin concentrations expressed by raw data (d), those corrected by BMI (e) and those corrected by body fat mass (f) in 136 patients with RA and 78 controls are shown. The horizontal line indicates the median value in each group. Wilcoxon rank sum test was used for the statistical analyses. P < 0.05 was considered statistically significant.

Article Snippet: EIA for adiponectin was performed in a similar manner, using anti-human adiponectin antibodies (10-7816 as the detection antibody and 10-7815 as the capture antibody) purchased from Fitzgerald Industries International (Acton, MA, USA).

Techniques: Comparison